Fig. 1.

Expression of macrophage specific markers. Splenocytes were clear of red blood cells by lysis and enriched for myeloid and DC subsets via T and B cell depletion. Cells were then stained with fluorochrome-conjugated antibodies specific for CD11b (PE-Cy7), CD11c (APC), Ly6C (FITC), Ly6G (PE), along with biotinylated antibodies to CD68, MOMA-1, SIGNR1 and F4/80. APC-Cy7-streptavidin was used as a secondary conjugate. L-DC, dendritic and myeloid subsets were gated as described in Table 1 and Hey et al., (2016) [10]. a Expression of CD68, MOMA-1, SIGNR1 and F4/80 on inflammatory monocytes (Infl mono), eosinophils (Eos), neutrophils (Neu) and macrophages (Macro). b Expression of CD68, MOMA-1, SIGNR1 and F4/80 on resident monocytes (Resi mono), L-DC and cDC subsets. Data are reflective of three independent analyses