Table 1.

RNA sequencing quality and coverage of soybean transcriptome

	Replicate	Total Reads	Q30 Bases (%)	Mapped Reads	Uniquely Mapped	Gene Coverage (%)
Conrad	1	43642500	92.91	30762485	24744536	64.28
	2	51423168	92.58	36648290	29163761	64.34
	3	41426398	92.67	29497436	23948533	63.96
AC Colombe	1	64237226	92.88	43062343	33956444	65.09
	2	41552636	92.65	29416157	23831591	63.96
	3	50795644	92.41	33359756	27313027	64.41
AC Glengarry	1	33146096	93.52	23522302	19393241	63.34
	2	49338208	93.54	34540611	27773084	64.27
	3	71279056	93.47	49674464	38962472	64.44
Pagoda	1	19820794	93.53	14005991	11582472	62.74
	2	42622034	93.45	30545146	24479762	63.26
	3	40106364	93.67	27711116	22598173	63.64

Coverage of the soybean transcriptome (Wm82.a2.v1) in soybean roots of Conrad, AC Colombe, AC Glengarry and Pagoda biological replicates ranged from 62.74 to 65.09%. Library replicate: the soybean cultivar replicate sequenced; total reads: the total number of sequence reads obtained; Q30 bases (%): the percentage of bases with > 30 quality after 3′ end trimming; mapped reads: the number of reads that mapped to one of the soybean transcript models; uniquely mapped reads: number of mapped reads mapping uniquely to a transcript model with a mapping quality ≥ 20; genes hit: number of gene models with one or more reads mapped to its transcript as a percentage of the total number of gene models (56,044 protein-coding loci)