Table 2.
Differentially expressed genes that are upregulated (p < 0.001) in high isoflavonoid cultivars: DE gene models were inspected manually using annotations from the soybean database (Phytozome), and the ‘GO’ annotations associated with their Arabidopsis homologs (TAIR); they were compiled into families for consideration based on function, and potential involvement in the phenylpropanoid pathway and as underlying factors for isoflavonoid content
| Log2 Fold Change | Predicted Cellular Compartment, Biological Processes and Molecular Functions | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Glyma ID | AC Colombe vs. | Conrad vs. | Gene Product Description | Arabidopsis Homolog | Cellular Compartment | Metabolism | Transport | Transcriptional Regulation | Stress Response | ||
| AC Glengarry | Pagoda | AC Glengarry | Pagoda | ||||||||
| Glyma.03G065700.1 | 6.72 | 6.14 | 7.13 | 6.58 | GRAS family transcription factor | AT2G37650.1 | nucleus | Transcription factor; development | light stress; signal transduction | ||
| Glyma.03G070300.1 | 1.35 | 1.66 | 1.53 | 1.86 | serine carboxypeptidase-like 19 | AT5G09640.1 | apoplast | specialized; sinapoyl-transferase activity; phenylpropanoid role | |||
| Glyma.06G213600.1 | 1.71 | 3.85 | 1.71 | 3.88 | Histone H3 K4-specific methyltransferase SET7/9 family protein | AT4G17080.1 | nucleus/cytoplasm | methyl-transferase | histone modification | ||
| Glyma.06G268600.1 | 2.59 | 3.06 | 2.85 | 3.34 | disease resistance protein (TIR-NBS-LRR class) | AT5G17680.1 | nucleus/cytoplasm | defense response; signal transduction; cell death | |||
| Glyma.06G268700.1 | 5.06 | 5.27 | 5.17 | 5.38 | disease resistance protein (TIR-NBS-LRR class) | AT3G25510.1 | nucleus/cytoplasm | defense response; signal transduction; cell death | |||
| Glyma.06G308400.1 | 3.95 | 4.50 | 3.35 | 3.92 | alpha/beta-hydrolases superfamily protein | AT5G06570.1 | nucleus | carboxylic ester hydrolase activity | amino acid transport | ||
| Glyma.08G087100.1 | 2.90 | 2.97 | 2.54 | 2.62 | thioredoxin-1 | AT2G35010.1 | cytoplasm | oxidoreductase activity; acts on sulfur group | cell redox homeostasis; symbiosis | ||
| Glyma.10G029100.1 | 4.29 | 4.61 | 4.13 | 4.45 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | AT3G63290.1 | plasmo-desmata | oxidoreductase activity; pollen development | |||
| Glyma.11G037100.1 | 2.48 | 4.11 | 2.63 | 4.28 | FAD/NAD(P)-binding oxidoreductase family protein | AT2G35660.1 | chloroplast/mitochondria | monooxygenase activity | |||
| Glyma.12G188200.1 | 5.12 | 7.20 | 4.60 | 6.67 | histone deacetylase 8 | AT1G08460.1 | nucleus | histone deacetylase; H3K14 specific; NAD dependent | |||
| Glyma.17G144300.1 | 1.37 | 1.41 | 1.09 | 1.14 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | AT4G16770.1 | cytoplasm/peroxisome | oxidoreductase activity; flavonoid biosynthetic process | |||
| Glyma.17G165600.1 | 1.01 | 1.13 | 0.67 | 0.80 | zinc finger protein 7 | AT1G24625.1 | nucleus | DNA-templated transcriptional regulation; | |||
The log2 fold changes for the gene models in each of the four pair-wise comparisons between cultivars are detailed in the table