Figure 5. Photographs and histological results showing vessel disruption after PUT treatment.

(A) The photograph of a control rabbit ear treated with either laser (circled area) or ultrasound (squared area) only. 1 MHz ultrasound-only at 0.6 MPa negative peak pressure (control) and with laser-only at 20 mJ/cm² fluence at 584 nm (control). (B) The photograph of a rabbit ear after PUT treatment with 0.6 MPa ultrasound negative peak pressure and 20 mJ/cm² laser fluence at 584 nm. Disruption of capillaries can be clearly noticed. (C–F) Histological photographs (H&E stain) showing the changes after the PUT treatment. (C) Shows detached endothelial cells from vessel wall (white arrow) and extravasation of erythrocytes (black triangle). (D) Shows disappearance of endothelial cells (white arrows). (E) Shows intravascular thrombus (black arrow). (F) Shows intravascular fibrin clot within the lumen and surrounding adherent red blood cells.