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. 2017 Jan 11;17:59. doi: 10.1186/s12879-016-2104-z

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Detection of IgG1 and IgG2a levels in immunized mice sera by ELISA. Sera was collected at 2 weeks after the last injection and detected by ELISA. All samples were performed four times. The results are mean of 27 mice per group and expressed as the mean of the optical density of 490 ± SD. * P < 0.05, as compared with PBS and pEGFP-C1; # P < 0.05, as compared with ROM4 or peptide; & P < 0.05, as compared with pSAG1