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. 2017 Jan 11;7:40279. doi: 10.1038/srep40279

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Copyright © 2017, The Author(s)

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(A) The flower, (B) Mature pollen grains, and (C) Pollen tube showed strong SEC3A expression. (D) Flowers at stage 9, 11, 12 were used for anther dissection. (E) DAPI staining showed that pollen from stage 9, 11, 12 flowers was at unicellular, bicellular, and the tricellular stages, respectively. (F) GUS staining of anthers from flowers of (D). (G) A schematic representation of SEC3A transcript and the site of T-DNA insertion. The T-DNA insert is located in the last Exon. The black boxes represent exons, lines in between represent introns. GKLB, P1, P2 are the primers used in the PCR assays. (H) Genotyping of sec3a/SEC3A by PCR analysis. TUB8 was used as an internal control. Bars = 0.2 mm for (A,F), 10 μm for (B,C), 0.4 mm for (D), and 5 μm for (E).