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. 2017 Jan 11;7:40279. doi: 10.1038/srep40279

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(A,B) Calcofluor white staining. (C,D) Pollen grains labeled with anti-callose monoclonal antibody. (E,F) Pollen grains labeled with JIM5 monoclonal antibody. (G,H) Pollen grains labeled with JIM7 monoclonal antibody. sec3a pLAT52:SEC3A-GFP (A,C,E,G) and sec3a (B,D,F,H) pollen were used for the assays. (I–L) Ruthenium red staining of tetrads from qrt1/qrt1 (I,K) and sec3a/SEC3A qrt1/qrt1 (J,L) plants. Bars = 10 μm.