Viability of cryopreserved human adipose-derived mesenchymal stem cells (ASCs). Human ASCs (passages 4–8) cryopreserved in four different solutions, that is, 10% dimethyl sulfoxide (DMSO), Cell Banker 2, Cell Banker 3, and TC protector, were assayed for cell viability. The cell populations were cryopreserved for 7 days. Immediately after thawing of the cells, cell viability was evaluated using the trypan blue exclusion procedure. The averages of three independent assays are indicated with SE bars.