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. 2004 Oct;3(5):1154–1163. doi: 10.1128/EC.3.5.1154-1163.2004

FIG. 1.

FIG. 1.

Localization of CgNdc10-HA, CgCep3-HA, and CgCtf13-HA to C. glabrata CEN DNA by ChIP. C. glabrata strains CgTS10 (Cgndc10), CgTS12 (Cgcep3), and CgTS11 (Cgctf13) bearing plasmids expressing HA-tagged versions of CgNDC10, CgCEP3, and CgCTF13 (pTS130, pTS131, and pTS124; see Table S1 in the supplemental material for plasmid constructs) were grown to late logarithmic stage. After in vivo cross-linking, extracts of these strains were immunoprecipitated (IP) with anti-HA antibody. Aliquots of the extracts (the load was 0.05 μl of chromatin solution, with the exception of CgCtf13-HA, for which the load was 0.025 μl) and the immunoprecipitates (2 μl of chromatin solution, except for CgCtf13-HA, for which 0.2 μl was used) were analyzed by PCR. PCR mixtures (see Table S2 in the supplemental material for primers) were designed to amplify the CEN DNA fragment in addition to two noncentromeric control fragments (ACT1 and LEU2).