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Cep3p-Ctf13p interactions in a yeast two-hybrid system. Shown is the interaction of truncated GAD-CgCep3p with full-length GBD-CgCtf13p. Truncated versions of CgCep3p were generated by restriction digestion of pTS101 or by PCR (see Table S1 in the supplemental material). Symbols: +++, strong interaction, with growth on SD medium lacking Ade and lacking His and blue colony color; −, no interaction.
