FIG. 4.

Lithium inhibits regeneration of flagella. (A-D) Histograms (n = 105 to 111 cells per measurement) show distribution of flagellar lengths before and after regeneration. Wild-type cells were preincubated without (A, C) or with (B, D) 10 μg of cycloheximide/ml. After 10 min, 25 mM LiCl was added (C, D) and and the incubation was continued for an additional 60 min. Arrows indicate the average lengths of flagella for each cell population. (E) RNA blot analysis of flagellar transcripts during regeneration. Twenty micrograms of total RNA from either flagellated cells (lanes F) incubated without (Control) or with LiCl and cells regenerating flagella (lanes R) in the absence (Control) or presence of LiCl was loaded in each lane. The transcripts for RSP3 and PF20 were detected by a hybridization probe from the corresponding gene. Blots were reprobed with CRY1 (the gene encoding ribosomal protein S14) to demonstrate equal loading. A faint second message that is unaffected by LiCl was identified with the PF20 probe.