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Phenotypes of thiamine-deficient mutants of Lotus japonicus. Photographs show the pods, leaves, and nodules of the wild-type (WT) plant and the thiamine-deficient thi1 and thiC mutants. The thi1 mutant showed abnormal phenotypes in pods and nodules, and the thiC mutant showed chlorosis. Although thiamine did not affect AM in our previous study, 3 further investigations will be required to understand its function in AM.
