FIG. 2.

Rapamycin and Msn2p regulation. A. Rapamycin and Msn2 localization. Wild-type strain AKY34 containing the full-length Msn2-myc₁₂ fusion was grown at 30°C to mid-log phase and transferred to 30°C medium, 30°C medium containing 200 ng of rapamycin (rap) per ml, or 38°C medium. Cells were monitored by epifluorescence and phase-contrast light microscopy after 0, 5, 10, 15, 30, and 60 min (pictures shown are from 30 min). B. Rapamycin and PUT1 expression. The put1-lacZ fusion strain AKY35 was transferred to 30°C medium containing 200 ng of rapamycin (Rap) per ml for the indicated times, and β-galactosidase (β-Gal) activity was measured.