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. 2004 Oct;3(5):1261–1271. doi: 10.1128/EC.3.5.1261-1271.2004

FIG. 3.

FIG. 3.

The Tap42p-interacting protein Tip41p influences Msn2p localization. A. tip41Δ prevents Msn2p nuclear accumulation in a tap42(Ts) mutant. Strains containing full-length Msn2-GFP fusion were grown at 23°C to mid-log phase and transferred to 36°C medium. After 30 min, cells were viewed by epifluorescence and phase-contrast light microscopy. In panels A and B, strains AKY79 (TAP42 TIP41), AKY84 [tap42-106(Ts) TIP41], AKY142 (TAP42 tip41Δ), and AKY144 [tap42-106(Ts) tip41Δ] are shown. B. tip41Δ and tap42(Ts) growth. Strains from panel A were incubated for 3 days at 23 and 30°C. C. Msn2 and tap42(Ts) growth. Strains were incubated for 3 days at 23, 30, and 36°C. Strains Y3033 (TAP42 MSN2,4), Y3036 (TAP42 msn2Δ,4Δ), Y3034 (tap42-106 MSN2,4), Y3037 (tap42-106 msn2Δ,4Δ), Y3034 (tap42-109 MSN2,4), Y3038 (tap42-109 msn2Δ,4Δ) are shown. D. tip41Δ prevents Msn2p nuclear accumulation in response to rapamycin. Strains were grown at to mid-log phase at 23°C and transferred to 36°C medium either lacking or containing rapamycin (200 ng/ml). Strains AKY36 (TAP42 TIP41) and AKY75 (TAP42 tip41Δ) are shown.