FIG. 4.

PP2A activity and Msn2p translocation in response to rapamycin and stress. A. PP2A catalytic activity is required for Msn2p translocation in response to rapamycin. Strains containing the Msn2-myc₁₂ fusion were grown to mid-log phase at 23°C and transferred to 36°C medium lacking or containing 200 ng of rapamycin per ml. After 30 min, cells were viewed by epifluorescence and phase-contrast light microscopy. Strains AKY101 (wild type [WT]) and AKY103 (pph21Δ pph22Δ) are shown. B. PP2A catalytic activity is required for Msn2p translocation in response to stress. Strains shown in panel A were grown to mid-log phase in medium containing 2% glucose and then transferred to the same medium, the same medium containing 1 M sorbitol, or the same medium lacking glucose. After 30 min (10 min for glucose depletion), cells were visualized by epifluorescence, phase-contrast light, and differential-contrast (DIC) microscopy.