FIG. 2.

Abnormal cell morphology and septum formation of the cps5-138 mutant under restrictive culture conditions. Wild-type and cps5-138 mutant cells were stained with calcofluor. Log-phase cells of the wild type (a) and cps5-138 mutant (b) cultured in YE medium at 28°C and wild-type (c) and cps5-138 mutant (d) cells in EMM at 28°C. Log-phase cells of the wild type (e) and cps5-138 mutant (f) were transferred to Ca²⁺-free EMM and cultured for 3 h at 28°C. Wild-type (g) and cps5-138 mutant (h) cells cultured in YE medium at 35°C for 15 h. Wild-type (i) and cps5-138 mutant (j) cells cultured in YE medium plus CsA (10 μg/ml) at 28°C for 15 h. Bar, 5 μm.