FIG. 3.

Defective cytokinesis of the cps5-138 mutant under restrictive culture conditions. The cps5-138 mutant was cultured in YE medium at 28°C to the log phase (a), and the collected cells were transferred to Ca²⁺-free EMM and cultured for 3 (b) and 6 (c) h. Each cell culture was stained with propidium iodide (57) and analyzed with an LSR flow cytometer (BD Biosciences). The horizontal and vertical axes show relative DNA content and cell number, respectively.