FIG. 5.

Effects of MnCl₂ on cell growth and morphology of the wild-type (WT), cps5-138, and cps5Δ strains. A: Each strain was cultured in Ca²⁺-free EMM and Ca²⁺-free EMM containing 0.5 or 5.0 mM MnCl₂. After 48 h of cultivation, the optical density (O.D.) at 600 nm was measured to quantify the cell growth. B: The cps5-138 cells were grown in Ca²⁺-free EMM or Ca²⁺-free EMM containing 0.5 mM MnCl₂ to the log phase and observed with a Nomarski interference microscope. w/o, without.