FIG. 6.

Intracellular localizations of GFP-tagged Pmr1p/Cps5p, Alg3p/Cps11p, and Pmc1p. Pmr1p was observed in cps5Δ cells capable of growing in Ca²⁺-free EMM with multicopy plasmids bearing the fused gene or the gene integrated in the chromosome. Almost the same fluorescence pattern was obtained in each case. The localizations of Alg3p and Pmc1p were examined in each disruptant with multicopy plasmids. Pmr1p-GFP (top panel) and Alg3p-GFP (middle panel) display a localization pattern typical of S. pombe ER proteins, and Pmc1p-GFP (bottom panel) shows the vacuole membrane localization. Bar, 5 μm.