FIG. 9.

Complementation of the high-Ca²⁺-sensitive cell growth of the pmc1Δ strain. A: The cell suspension from the pmc1Δ strain transformed with pAL-KS, pREP1, pAL-KS bearing pmc1⁺-GFP, or pREP1 bearing cps5⁺ was spotted on an EMM plate containing 100 mM CaCl₂. B: The pmc1Δ cell suspension was spotted on a YE plate containing 100 mM CaCl₂ or 100 mM CaCl₂ plus CsA (10 μg/ml). The spotted plates were incubated at 28°C for 4 to 5 days.