TABLE 2.
Characterization of (1,3)-β-d-glucan synthase activities from S. pombe wild-type, cps5-138, and cps5Δ strains grown at 28 and 37°C in the absence or presence of 1.2 M sorbitol
| Growth temp (°C) | Strain | Sp act (mean ± SD)a |
|---|---|---|
| 28 | Wild type | 10.1 ± 0.1 (100) |
| cps5-138 | 5.7 ± 0.2 (56) | |
| cps5Δ | 4.5 ± 0.2 (45) | |
| 28 + sorbitol | Wild type | 5.8 ± 0.2 (100) |
| cps5-138 | 3.3 ± 0.4 (57) | |
| cps5Δ | 2.3 ± 0.2 (40) | |
| 37b | Wild type | 4.6 ± 0.1 (100) |
| cps5-138 | 5.7 ± 0.0 (124) | |
| cps5Δ | 6.8 ± 0.7 (148) | |
| 37 + sorbitol | Wild type | 6.1 ± 0.1 (100) |
| cps5-138 | 5.5 ± 0.1 (90) | |
| cps5Δ | 5.2 ± 0.1 (85) |
a
Specific activity is expressed as milliunits per milligram of protein. The reaction mixture contained 150 μM GTP. The values were obtained from three independent experiments. Values in parentheses are percentages of specific activity compared to that of the wild type under the same conditions.
b
The cells were grown for 24 h at 37°C. Under these conditions, the thermosensitive morphological phenotype is completely expressed in the absence of sorbitol, and the cells are still viable and able to grow.