TABLE 3.
Characterization of (1,3)-β-d-glucan synthase activities from S. pombe wild-type; cps1-12, cps5-138, and cps5Δ mutant; and cps1-12 cps5-138 double mutant strains grown at 28°C in EMM in the presence or absence of calcium
| CaCl2, concn (mM) | Strain | Sp act (mean ± SD)a |
|---|---|---|
| 10 | Wild type | 7.4 ± 0.4 (100) |
| cps1-12 | 8.7 ± 0.7 (118) | |
| cps5-138 | 6.6 ± 0.6 (89) | |
| cps5Δ | 6.1 ± 0.4 (82) | |
| cps1-12 cps5-138 | 6.2 ± 0.5 (84) | |
| 0.1 | Wild type | 9.8 ± 0.1 (100) |
| cps1-12 | 12.4 ± 1.2 (127) | |
| cps5-138 | 8.7 ± 1.2 (89) | |
| cps5Δ | 6.2 ± 0.8 (63) | |
| cps1-12 cps5-138 | 8.0 ± 0.5 (82) | |
| 0b | Wild type | 15.0 ± 0.4 (100) |
| cps1-12 | 12.3 ± 0.9 (82) | |
| cps5-138 | 8.0 ± 0.7 (53) | |
| cps5Δ | 7.6 ± 1.3 (51) | |
| cps1-12 cps5-138 | 12.6 ± 1.1 (84) |
a
Values were obtained from three independent experiments as for Table 2. Values in parenthesis are percentages of specific activity compared to that of the wild type under the same conditions.
b
The cells were grown for 4 h in Ca2+-free EMM plus 0.1 mM EGTA. A longer incubation time produced the appearance of some cell lysis in the mutant cultures.