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. 2004 Oct;3(5):1124–1135. doi: 10.1128/EC.3.5.1124-1135.2004

TABLE 4.

Incorporation of radioactivity from [14C]glucose into cell wall polysaccharides of S. pombe wild-type, cps5-138, and cps5Δ strains grown at 28°C in EMM in the presence or absence of calcium

CaCl2 Concn (mM) Strain % Incorporation of [14C]glucose (mean ± SD)a
Cell wall Galactomannan α-Glucan β-Glucan
10 Wild type 34.3 ± 0.7 4.8 ± 0.2 (14.0) 9.0 ± 0.4 (26.2) 20.5 ± 0.1 (59.8)
cps5-138 34.0 ± 1.2 3.4 ± 0.1 (10.0) 8.0 ± 0.5 (23.5) 22.6 ± 0.6 (66.5)
cps5Δ 34.1 ± 0.7 3.4 ± 0.2 (10.0) 8.2 ± 0.0 (24.0) 22.5 ± 0.9 (66.0)
0b Wild type 34.3 ± 0.8 3.7 ± 0.0 (10.8) 9.1 ± 0.3 (26.5) 21.5 ± 0.9 (62.7)
cps5-138 37.4 ± 1.2 <0.01 (0) 12.8 ± 0.6 (34.2) 24.6 ± 0.6 (65.8)
cps5Δ 40.8 ± 0.8 <0.01 (0) 13.7 ± 0.4 (33.6) 27.1 ± 0.4 (66.4)
a

Percent incorporation of [14C]glucose is the counts per minute incorporated per fraction × 100/total counts per minute incorporated. The values were obtained from three independent experiments. Values in parentheses are percentages of the corresponding polysaccharide in the cell wall composition.

b

The cells were grown for 4 h in Ca2+-EMM plus 0.1 mM EGTA.