TABLE 4.
CaCl2 Concn (mM) | Strain | % Incorporation of [14C]glucose (mean ± SD)a
|
|||
---|---|---|---|---|---|
Cell wall | Galactomannan | α-Glucan | β-Glucan | ||
10 | Wild type | 34.3 ± 0.7 | 4.8 ± 0.2 (14.0) | 9.0 ± 0.4 (26.2) | 20.5 ± 0.1 (59.8) |
cps5-138 | 34.0 ± 1.2 | 3.4 ± 0.1 (10.0) | 8.0 ± 0.5 (23.5) | 22.6 ± 0.6 (66.5) | |
cps5Δ | 34.1 ± 0.7 | 3.4 ± 0.2 (10.0) | 8.2 ± 0.0 (24.0) | 22.5 ± 0.9 (66.0) | |
0b | Wild type | 34.3 ± 0.8 | 3.7 ± 0.0 (10.8) | 9.1 ± 0.3 (26.5) | 21.5 ± 0.9 (62.7) |
cps5-138 | 37.4 ± 1.2 | <0.01 (0) | 12.8 ± 0.6 (34.2) | 24.6 ± 0.6 (65.8) | |
cps5Δ | 40.8 ± 0.8 | <0.01 (0) | 13.7 ± 0.4 (33.6) | 27.1 ± 0.4 (66.4) |
Percent incorporation of [14C]glucose is the counts per minute incorporated per fraction × 100/total counts per minute incorporated. The values were obtained from three independent experiments. Values in parentheses are percentages of the corresponding polysaccharide in the cell wall composition.
The cells were grown for 4 h in Ca2+-EMM plus 0.1 mM EGTA.