TABLE 4.
Incorporation of radioactivity from [14C]glucose into cell wall polysaccharides of S. pombe wild-type, cps5-138, and cps5Δ strains grown at 28°C in EMM in the presence or absence of calcium
| CaCl2 Concn (mM) | Strain | % Incorporation of [14C]glucose (mean ± SD)a
|
|||
|---|---|---|---|---|---|
| Cell wall | Galactomannan | α-Glucan | β-Glucan | ||
| 10 | Wild type | 34.3 ± 0.7 | 4.8 ± 0.2 (14.0) | 9.0 ± 0.4 (26.2) | 20.5 ± 0.1 (59.8) |
| cps5-138 | 34.0 ± 1.2 | 3.4 ± 0.1 (10.0) | 8.0 ± 0.5 (23.5) | 22.6 ± 0.6 (66.5) | |
| cps5Δ | 34.1 ± 0.7 | 3.4 ± 0.2 (10.0) | 8.2 ± 0.0 (24.0) | 22.5 ± 0.9 (66.0) | |
| 0b | Wild type | 34.3 ± 0.8 | 3.7 ± 0.0 (10.8) | 9.1 ± 0.3 (26.5) | 21.5 ± 0.9 (62.7) |
| cps5-138 | 37.4 ± 1.2 | <0.01 (0) | 12.8 ± 0.6 (34.2) | 24.6 ± 0.6 (65.8) | |
| cps5Δ | 40.8 ± 0.8 | <0.01 (0) | 13.7 ± 0.4 (33.6) | 27.1 ± 0.4 (66.4) | |
a
Percent incorporation of [14C]glucose is the counts per minute incorporated per fraction × 100/total counts per minute incorporated. The values were obtained from three independent experiments. Values in parentheses are percentages of the corresponding polysaccharide in the cell wall composition.
b
The cells were grown for 4 h in Ca2+-EMM plus 0.1 mM EGTA.