Identification of mutations that suppress Wallerian degeneration. (A) ORN MARCM clones in control, l(3)896, l(3)4621, and l(3)4705. Right, axotomy; left, uninjured control. n ≥ 15 brains. (B) Control and l(3)896 brains 30 days after injury. n ≥ 10 brains. (C) l(3)896 clones 50 days after injury. n = 11 brains. (D) MARCM clones in mushroom body (MB) γ neurons in control and l(3)896 backgrounds at the indicated developmental stages. dorsal (d) and medial (m) axonal branches (arrows), and dendrites (circled). n ≥ 15 brains for all. (E) dMP2 neurons with GFP. Ventral views (anterior up) of stage 16 embryos (left) and first-instar larvae. dMP2 neurons before (arrows) and after (arrowheads) segment-specific apoptosis. n ≥ 20 flies at each time point. (F) Wild-type and l(3)896 mutant clones with (right) or without (left) ectopic expression of hid. n ≥ 20 flies.