Sarm1−/− neurons in primary culture are protected from Wallerian degeneration. (A) Phase contrast images of SCG explant cultures from wild-type (top), Sarm−/− (middle), and WldS-expressing (bottom) animals at the indicated time after axotomy. (B) Quantification from (A). Mean T SEM, *P < 0.01. (C) Axon preservation at the indicated time points in cortical neuron cultures from E16.5 mouse embryos. α-Tau, red. (D) Quantification from (C). Mean T SEM, *P < 0.01. (E) Axon preservation at the indicated time points in DRG cultures from E13.5 mouse embryos. α-TUJI, green. (F) Quantification from (E). Mean T SEM, *P < 0.01.