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. 2004 Oct;3(5):1164–1168. doi: 10.1128/EC.3.5.1164-1168.2004

FIG. 3.

FIG. 3.

Stability measurements of Sec20p. (A) Pulse-chase experiments were carried out using strain CAI4(pYW69) (Sec20-myc) and, as a control, strain CAI4(pYW7) (Sec20). Cells were labeled with TRAN35S-LABEL (ICN) for 15 or 45 min (pulse), followed by an excess of methionine-cysteine and further incubation (chase). At 30 or 60 min of chase, samples were immunoprecipitated, followed by SDS-PAGE and autoradiography. (B) Strains CAI4(pYW69) (wt) and CAP1-3121(pYW69) (pmt1) were examined by pulse-chase measurements as for panel A; strain CAI4(pYW7) (Sec20) was used as the control. The migration of a nonspecifically immunoprecipitated protein is labeled by the asterisk.