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. 2016 Dec 20;8(4):6791. doi: 10.4081/rt.2016.6791

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©Copyright C.L. Brett et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — The Hematoxylin and Eosin (H&E) section of the tumor cells demonstrated the proliferation of spindle cells eroding the overlying squamous mucosa (a, H&E 4×). Focal necrosis is noted (b, H&E 10×). The spindle cells showed marked cytological atypia and brisk mitoses (c, H&E 20×). FISH study on the paraffin block demonstrated CPM gene amplification (orange) in tumor cells (d, green labeled the probe for chromosome 12 centromere; orange labeled the probe for CPM gene at 12q15; aqua labeled the probe for chromosome 4 centromere as internal control).