Fig. 2.

Overall structure of human IgG4-Fc solved at room temperature from crystals in situ. (A) Electron density for the oligosaccharide moiety from chain A. A 2F_o-F_c map is shown, contoured at 1σ. The oligosaccharide residues are labelled as follows: BMA, β-d-mannose; NAG, N-acetylglucosamine; MAN; α-d-mannose, FUC, α-l-fucose. (B) Overall structure of human IgG4-Fc. Chains A and B for the room temperature structure are colored blue and purple, respectively. For comparison, the cryogenic IgG4-Fc structure (Davies et al., 2014b) is shown in gray. The disposition of the Cγ2 domain from chain A differs when the two structures are superposed (RMSD 0.36 Å for chain B Cγ3 domain Cα atoms). The positions of the Cγ2 BC and FG loops are indicated for chain B. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)