Table 1.
Data collection, processing and refinement statistics.
| Data collection | |
| Number of crystals used | 48 |
| Number of datasets collected | >200 partial datasets |
| Temperature (K) | 293 |
| Data processing | |
| Space group | P 212121 |
| Unit cell dimensions | |
| a, b, c (Å) | 73.29, 81.93, 103.88 |
| Resolution (Å) | 48.35−2.70 (2.83−2.70)a |
| Completeness (%) | 99.9 (99.9)a |
| Multiplicity | 8.7 (8.9)a |
| Mean ((I)/σ(I)) | 12.2 (2.0)a |
| CC1/2 | 0.992 (0.433)a |
| Rmerge (%) | 17.1 (288.4)a |
| Rpim (%) | 6.0 (98.9) a |
| Wilson B factor (Å2) | 77.7 |
| Refinement | |
| Rwork/Rfree (%)b | 17.53/23.13 |
| RMSD | |
| Bond lengths (Å) | 0.003 |
| Bond angles (°) | 0.540 |
| Coordinate error (Å) | 0.39 |
| No. of atoms | |
| Proteinc | 3270 |
| Oligosaccharide A/B | 99/99 |
| Solvent | 20 |
| Ave. B factor (Å2) | |
| Protein: Cγ2 A/B | 74.9/115.3 |
| Protein: Cγ3 A/B | 72.3/69.8 |
| Oligosaccharide A/B | 86.8/143.3 |
| Solvent | 60.6 |
| Ramachandran plotd | |
| Favoured (%) | 98.6 |
| Allowed (%) | 100 |
a
Values in parentheses are for the highest resolution shell.
b
Rfree set comprises 5% reflections.
c
Includes alternative conformations.
d
Ramachandran plot generated by MolProbity (Chen et al., 2010).