Figure 3.

Mutant α1β2γ2L receptors showed decreased GABA-evoked whole-cell currents and increased zinc sensitivity. (A) Representative GABA current traces are shown that were obtained following rapid application of 1 mM GABA for 4 s to lifted HEK293T cells voltage clamped at −20 mV. (B and C) Bar graphs showing average peak current and zinc inhibition from cells co-expressing α1β2 subunits with wild-type (wt) or mutant γ2 subunits. Values are expressed as mean ± SEM (Supplementary Table 1). One-way ANOVA with Dunnett’s post-test was used to determine significance compared to the wild-type condition. ****P < 0.0001, ***P < 0.001, and ^nsP > 0.05, respectively.