FIG. 2.

Countin does not affect the level of Akt/PKB protein during development. (A) Vegetative and 6-h-starved Ax4 and countin⁻ cells were collected, and Western blots of the total cell lysates were stained with anti-Akt/PKB antibodies. The results are representative of three independent experiments. (B) Western blots of total cell lysates from cells treated without (control) or with anti-countin antibodies were stained with anti-Akt/PKB antibodies. (C) Western blots of the NP-40-soluble fractions of the cell lysates were stained with anti-Akt/PKB antibodies. The difference in band intensities compared to bands shown in panel B is due to a difference in the exposure of the X-ray film to the chemiluminescence-stained Western blot.