Figure 2.

Bayesian phylogenies of flaviviruses over the whole open reading frame nucleotide sequence. The tree was constructed in Geneious using MrBayes v3.2.2 under the Bayesian Marko chain Monte Carlo (MCMC) model with a general time reversible substitution model, gamma distribution (five discrete gamma categories), and invariant rates among sites. Horizontal branch lengths represent posterior probabilities. The tree has been rooted using the outgroup Modoc virus (MODV), a flavivirus with no known vector. The colored nodes represent insect-specific flaviviruses (ISFs), with Lineage I in blue and Lineage II in red.