Figure 3.

Metformin increases cell death in ACHN cells. ACHN cells were treated with 5 and 20 mM metformin for 48 hours, while an equal volume of phosphate-buffered saline (PBS) was used as vehicle control. (A) Cells were subjected to analysis of apoptosis with the FITC-Annexin V/PI apoptosis detection kit. Representative data from triplicate experiments are shown. (B) Quantification of the percentage of dead cells was derived from three independent experiments. Data show mean values ±SD from three independent experiments. * – Indicates p<0.05 compared with the control.