| Antibody Name | Concentration |
| SSEA4 | 1:200 |
| Oct3/4 | 1:100 |
| TRA-1-60 | 1:200 |
| Sox2 | 1:100 |
| TRA-1-81 | 1:100 |
| Klf4 | 1:250 |
| Alexa Fluor 488 goat anti-mouse IgG (H+L) antibody | 1:400 |
| Alexa Fluor 594 goat anti-rabbit IgG (H+L) antibody | 1:400 |
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