Therapeutic and chemo-preventive efficacies of VA extracts in a nude mice model. Animals (female, 5–6 weeks) were untreated or pretreated with VA extracts (20 mg/kg, high-dose or 10 mg/kg, low-dose) subcutaneously each day for ten days before a single subcutaneous injection of MDA-MB-468 cells (A) or Hras cells (B). The treatment groups were characterized and the protocols for measurements of tumor volumes are described in the Materials and Methods section. VA extracts can significant inhibit TNBC-derived tumor growth. Although there was a lag in each treatment group, pre-treatment with VA was the most efficacious, implicating VA extracts as chemo-preventive agents.