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. 2016 Jul 1;7(31):48860–48869. doi: 10.18632/oncotarget.10379

Figure 5. Both IL-12 and IFN contributes to immunogenic cell death following intravenous K3-SPG treatment.

Figure 5

A. C57BL/6 mice and Il12p40-Ifnar2 double deficient mice were inoculated s.c. with EG7 cells at day 0, treated intravenously (i.v.) with K3-SPG at days 7, 9, and 11, and then were sacrificed at day 12. Splenocytes were collected and stained by anti-CD45 antibody and then analyzed by flow cytometry. B. Scatter plots indicate CD45 negative cell populations. *p < 0.05 (t-test). (C.-E.) Il12 p40-, Ifnar2-hetero and deficient mice, and Il12p40-Ifnar2 double deficient mice were inoculated with EG7 s.c. into the right flank at day 0 and then were treated with PBS, or K3-SPG i.v. at days 7, 9, and 11. Error bars represent mean + SEM (n = 4). Arrows indicate timing of therapy. *p < 0.05 (t-test).