Figure 2. A strong nonsense mutation in the age-1 gene (allele mg44) reduces the recovery of many membrane proteins relative to wild-type controls, whereas feeding PIP₃ to worms restores some bands.

Polyacrylamide/SDS gels, stained with SYPRO Ruby after electrophoresis, show A. isolated membrane proteins, and B. proteins recovered from PIP₃-coated beads, bound after isolation of membrane proteins as in A.