Table 4. Survival data for PIP3-binding candidate knockdowns.
| Experiment 1 (N2) | Expt. 2 (N2) | Expt. 3 (N2) | Expt. 4 (N2) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| RNAi: | none | CAND-1 | RAD-50 | none | CAND-1 | none | RAD-50 | none | RAD-50 |
| Median | 18.5 | 23.5 | 23.5 | 23.5 | 28.5 | 22.5 | 25.5 | 22.5 | 22.5 |
| Ratio | — | 1.27 | 1.27 | — | 1.21 | — | 1.13 | — | 1.0 |
| Mean | 19.5 | 23.2 | 23.5 | 24.3 | 28.0 | 23.2 | 25.1 | 22.6 | 24.3 |
| Ratio | — | 1.19 | 1.20 | — | 1.15 | — | 1.10 | — | 1.08 |
| P < | — | 3E–5 | 3E–5 | — | 0.0001 | — | 0.007 | — | 0.008 |
| Expt. 5 (N2) RNAi from L4 | Experiment 6 (daf-16–) | Experiment 7 (daf-16–) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| RNAi: | none | CAND-1 | RAD-50 | none | CAND-1 | RAD-50 | none | CAND-1 | RAD-50 | |
| Median | 22.5 | 24.5 | 24.5 | 17.5 | 17.5 | 17.5 | 18.5 | 18.5 | 17.5 | |
| Ratio | — | 1.09 | 1.09 | — | 1.00 | 1.00 | — | 1.00 | 0.95 | |
| Mean | 23.5 | 24.5 | 24.6 | 18.9 | 18.6 | 18.4 | 18.5 | 18.6 | 18.5 | |
| Ratio | — | 1.04 | 1.05 | — | 0.99 | 0.97 | — | 1.00 | 1.00 | |
| P < | — | 0.04 | 0.06 | — | 0.6 | 0.4 | — | 0.8 | 1.0 | |
Median and Mean survivals are given in days post-hatch. Five independent experiments were performed in the wild-type N2-DRM strain, and two in strain SR814, created by outcrossing DR26 (daf-16(m26)) into N2-DRM. RNAi feeding began at hatch, or (Experiment 5) in late L4. Gehan-Wilcoxon
log-rank P values are shown.