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. 2016 Jun 30;7(31):48887–48904. doi: 10.18632/oncotarget.10346

Figure 2. Inhibition of rDNA transcription following amino acid depletion does not affect nucleolar integrity.

Figure 2

A. BJ-T cells (left panel) or HeLa cells (right panel) were starved of all amino acids (-AA) for the times indicated or amino acid and serum starved (-AAS) for 2 hours and then re-stimulated with all amino acids (AA) for 3 hours. Alternatively exponentially growing (EXP) HeLa cells were treated with 1 μM CX-5461 for 1 hour. Representative images of immunostaining for fibrillarin (FBL: Red) and nucleophosmin (NPM: Green) with DAPI (Blue) counterstain of n = 2. Scale bar, 10 μm. B. BJ-T cells or HeLa cells were treated as in A. and protein lysates analyzed by immunoblotting for the indicated antibodies. Representative images from n = 2-4 experiments.