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. 2016 Jul 19;7(31):49027–49041. doi: 10.18632/oncotarget.10717

Figure 1. urantide downregulates IRF3 expression induced by LPS/D-GalN in liver in ALF mice.

Figure 1

Mice were treated with urantide or vehicle 0.5 h before LPS/D-GalN injection. A. IRF3 mRNA expression in liver using RT-PCR assay. Left panel shows a representative ethidium bromide-stained gel of RT-PCR products, and right shows relative expression levels of IRF3 mRNA in liver after normalization to GAPDH. Data represent means ± SD (n = 6 each group). B. IRF3 mRNA expression in liver using real-time PCR. Relative expression levels of IRF3 mRNA were detected in liver after normalization to GAPDH through real-time PCR. Data represent means ± SD (n = 6 each group). C. IRF3 protein expression in liver using Western blot. Left panel shows a representative picture of Western blot, and right shows relative levels of IRF3 protein after normalization to GAPDH. Data represent means ± SD (n = 6 each group). *P<0.05 and **P<0.01 versus control mice [urantide(-)LPS/D-GalN(-)]; #P<0.05 and ##P<0.01 versus LPS/D-GalN-challenged mice [urantide(-)LPS/D-GalN(+)]. M, DL2000 DNA marker.