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. 2016 Jul 19;7(31):49027–49041. doi: 10.18632/oncotarget.10717

Figure 4. Interference adenovirus knockdowns IRF3 expression in LPS-stimulated KCs.

Figure 4

KCs were infected with IRF3 shRNA adenovirus 48 h before LPS stimulation. A. Manifestation of KCs after infected with adenovirus under microscopy. Left panel shows a representative picture of KC's morphology in light microscopy, and right shows GFP expression of KCs using fluorescence microscopy. B. IRF3 mRNA expression in KCs using real-time PCR. Relative expression levels of IRF3 mRNA were detected in KCs after normalization to GAPDH through real-time PCR. Data represent means ± SD (n = 6). C. IRF3 protein expression in the nuclear of KCs using Western blot. Left panel shows a representative picture of Western blot, and right shows relative levels of nuclear IRF3 protein after normalization to β-actin. Data represent means ± SD (n = 6). *P<0.05 and **P<0.01 versus control cells [adenovirus(-)LPS(-)]; #P<0.05 and ##P<0.01 versus LPS-stimulated cells [adenovirus(-)LPS(+)].