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. 2016 Jul 19;7(31):49027–49041. doi: 10.18632/oncotarget.10717

Table 2. Primer sequences used for real-time PCR.

Genes Primer sequences (5′→3′) Product size (bp)
IRF3
 Primer1 Sense 5′ AGGAGCTGTTAGAGATGG 3′ 103
Antisense 5′ TACTGGTCAGAGGTAAGG 3′
 Primer2 Sense 5′ACGCACAGATGGCTGACTTT3′ 102
Antisense 5′TCCTCTTCCAGGTTGACAGG3′
IFN-β Sense 5′GCTGAATGGAAGGCTCAAC3′ 105
Anti-sense 5′TGAATGGCAAAGGCAGTGTA3′
IL-1β Sense 5′GCCAACAAGTGGTATTCTCCA3′ 118
Anti-sense 5′CCGTCTTTCATCACACAGGA3′
TNF-α Sense 5′CAGGTTCCGTCCCTCTCATA3′ 100
Anti-sense 5′TGCCAGTTCCACATCTCG3′
GAPDH
 Primer1 Sense 5′ GTCGGTGTGAACGGATTTG 3′ 181
Antisense 5′ TCCCATTCTCAGCCTTGAC 3′
 Primer2 Sense 5′GACATGCCGCCTGGAGAAAC3′ 92
Antisense 5′GACATGCCGCCTGGAGAAAC3′