Table 2. Primer sequences used for real-time PCR.
Genes | Primer sequences (5′→3′) | Product size (bp) | |
---|---|---|---|
IRF3 | |||
Primer1 | Sense | 5′ AGGAGCTGTTAGAGATGG 3′ | 103 |
Antisense | 5′ TACTGGTCAGAGGTAAGG 3′ | ||
Primer2 | Sense | 5′ACGCACAGATGGCTGACTTT3′ | 102 |
Antisense | 5′TCCTCTTCCAGGTTGACAGG3′ | ||
IFN-β | Sense | 5′GCTGAATGGAAGGCTCAAC3′ | 105 |
Anti-sense | 5′TGAATGGCAAAGGCAGTGTA3′ | ||
IL-1β | Sense | 5′GCCAACAAGTGGTATTCTCCA3′ | 118 |
Anti-sense | 5′CCGTCTTTCATCACACAGGA3′ | ||
TNF-α | Sense | 5′CAGGTTCCGTCCCTCTCATA3′ | 100 |
Anti-sense | 5′TGCCAGTTCCACATCTCG3′ | ||
GAPDH | |||
Primer1 | Sense | 5′ GTCGGTGTGAACGGATTTG 3′ | 181 |
Antisense | 5′ TCCCATTCTCAGCCTTGAC 3′ | ||
Primer2 | Sense | 5′GACATGCCGCCTGGAGAAAC3′ | 92 |
Antisense | 5′GACATGCCGCCTGGAGAAAC3′ |