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. 2016 Apr 16;7(31):49122–49129. doi: 10.18632/oncotarget.8759

Figure 3. Functional analysis indicated that rs1447393 near miR-510 might protect from the risk of NOA.

Figure 3

A. RT-PCR was applied to detected mature miR-510 expression levels. The miR-510 expression level was significant down-regulated in mutant type compared with the wild type. B. Dual-luciferase reporter assay was conducted to measure the effects of rs1447393 near miR-510 on promoter-transcriptional activity. It was significantly increased in miR-510 mutant type for PRDX1. C. CCK8 was used to determine the influence of rs1447393 near miR-510 on cell growth. The cell growth was markedly increased with miR-510 mutant type at 48h. D, E. Flow cytometry assay was performed to analyze the effects of rs1447393 near miR-510 on cell apoptosis. (E) Histogram of cell apoptosis was presented to depict cell apoptotic percentages. There was no significant difference of cell apoptosis in rs1447393 near miR-510. F, G. Effects of rs1447393 near miR-510 on cell cycle were determined by flow cytometry. (G) Results quantitated in cell cycle were shown in histogram. There was no difference of cell cycle between miR-510 wild and miR-510 mutant type. Each data point represented the mean ± SE from three separate experiments in which treatments were performed in triplicate. *P<0.05, **P<0.01, ***P<0.001.