Figure 4. Changes of OncomiR target gene expression mediated by i-lncRNA.

A. SUDHL-4 cells were seeded into 24-well plates at a density of 1×10⁶ cells/100 μL/well and incubated with the indicated miRNA inhibitors at a concentration of 100 nmol/L for 48 h, the harvested cells were managed to detect the expressions of OncomiR target genes. GAPDH was used as the loading control. The densitometry analysis of every protein was performed, normalized with GAPDH content; **P<0.01 and ***P<0.001 compared with the corresponding parental cells. B. The four cell lines were planted in 24-well plates at a density of 1×10 ⁶ cells/100 μL/well for 24 h, then infected with Ad5F35-i-lncRNA or Ad5F35-EGFP at an MOI of 100 pfu/cell. After continuously cultured for 48 h, cells were harvested for isolation of total proteins, and the expressions of the indicated proteins were examined by Western blot. The densitometry analysis of every protein was performed, normalized with GAPDH content; **P<0.01 and ***P<0.001 compared with the corresponding parental cells.