Figure 5. Antitumor efficacy of i-lncRNA on DLBCL xenografts in mouse model.
A. SUDHL-4 cells were used to establish the xenograft tumor models. After tumors had developed, the mice were randomly assigned into three groups (Ad5F35-i-lncRNA group, Ad5F35-EGFP group, blank control group), 10 mice in every group. The virus treatment groups were received 5 injections of total viral dose of 1× 109 pfu/100 μL/mouse, one injection every other day. The blank control group was synchronously received injections of saline with same volume. After treatments, the tumor size was measured weekly and the tumor volume was calculated as “the maximum diameter × minimum diameter2 × 0.5” and used to plot growth curves; *P<0.05 and ***P<0.001 compared with the blank control group. B. The experimental observation was terminated at day 35 after the first treatment. The tumor specimens were harvested and weighed; ***P<0.001. C. The formalin-fixed, paraffin-embedded tumor sections were prepared for immunohistochemical staining to observe the expression of miRNA target gene expression, and the TUNEL assay was performed to evaluate cell apoptosis. For each slice, the positive cells were counted within 5 medium-power magnification fields of view (20× objective lens) under a microscope; original magnification: 200×; **P<0.01 and ***P<0.001 compared with the blank control group. D. The fresh tumor tissues were prepared to detect miRNA expression by qRT-PCR; *P<0.05, **P<0.01 and ***P<0.001 compared with the blank control group.