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. 2016 May 17;7(31):49156–49168. doi: 10.18632/oncotarget.9402

Figure 5. hCD24 regulates MET.

Figure 5

A. The mesenchymal cell line MiaPaCa2 was transiently transfected with an hCD24 expression vector. Cells were fixed after 48 hours and analyzed by immunofluorescence. Cells expressing hCD24 had an epithelial phenotype characterized by membranous β-catenin expression. B. Gene set enrichment analysis of pcDNA3-CD24 transfected MiaPaCa2 cells. 24 hours after transfection mRNA was isolated and analyzed using an Ilumina beadchip. Statistical analyses: FDRq < 0.05, nominal p-value < 0.05. C. MiaPaCa2 cells were transiently transfected with hCD24 for 24 hours. Left panel shows successful transfection. Right panel: analysis of mesenchymal transcription factors by real-time qRT-PCR. Mean ± SD. D. Working model: after an initial increase of CD24 expression in the pancreas, WNT signaling leads to β-catenin dephosphorylation and to a further increase of CD24 protein. Sustained CD24 expression upregulates β-catenin expression. E. Eventually CD24 accumulation at the cell surface stabilizes an epithelial phenotype.