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. 2016 Jun 21;7(31):49180–49193. doi: 10.18632/oncotarget.10205

Figure 2. Ablation of Mig-6 enhances EGF-induced cell migration.

Figure 2

H1299 and MCF-10A cells were infected with lentivirus encoding shRNA specific for Mig-6 (shMig-6) or a scrambled shRNA sequence (shC). (A) Cell lysates were subjected to western blotting, as indicated. (B) Wound-healing assays were performed in the presence of EGF for 12 hours. Wound closure data was quantitated by percentage of wound closure and presented as means and SE. *P < 0.05. (C) Stable H1299 or MCF10A cells were subjected to transwell assays in the presence of EGF for 8 hours (H1299) or 24 hours (MCF10A), respectively. Representative images are shown. Scale bars = 100 μm. (D) Results from three independent experiments in duplicate were quantitated and presented as means and SE ***P < 0.001. (E) Stable cells exhibiting filopodia were visualized by fluorescent microscopy. F-actin was visualized with phalloidin (red), and nuclei was counter-stained with DAPI (blue). Representative images (and insets) from three independent experiments performed in duplicate are shown. Scale bars = 50 μm. (F) Percentage of cells with multiple filopodia (defined as cells exhibiting at least 20 filopodia) were quantified and presented as means and SE from three independent experiments. *P < 0.05.