Skip to main content
. 2016 Jun 21;7(31):49180–49193. doi: 10.18632/oncotarget.10205

Figure 6. Mig-6 CRIB domain binding to Cdc42 is necessary and sufficient to inhibit cell migration independently of EGFR binding.

Figure 6

(A) H1299 cells and MCF-10A cells were stably infected with lentivirus expressing wild type (WT) or EGFR-binding deficient mutant Myc-tagged Mig-6(M346A). Whole cell lysates were subjected to western blotting as shown. (B) Stable H1299 and MCF-10A cells were subjected to transwell assays in the presence of EGF for 8 hours (H1299) or 24 hours (MCF10A), respectively. Results from three independent migration assays were quantitated and presented as means and SD. *P < 0.05. (C–D) H1299 cells stably expressing with CRIB or a quadruple mutant (CRIB-4A) were subjected to q-PCR analyses (C), or transwell assays in the presence of EGF for 8 hours. Representative images are shown (D). (F) Results from three transwell assays in duplicate were quantitated and presented as means and SD. ***P < 0. 001.