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. 2016 Jun 21;7(31):49180–49193. doi: 10.18632/oncotarget.10205

Figure 7. WT Mig-6, but not the 4A mutant, inhibits Cdc42 activation.

Figure 7

(A) H1299 cells stably expressing Mig-6-FL or FL-4A mutant were subjected to western blotting, as shown. (B) H1299 cells stably expressing CRIB or CRIB-4A were subjected to western blotting. (C) H1299 cells stably expressing shRNA specific for Mig-6 (shMig-6) or a scrambled shRNA sequence (shC) were subjected to western blotting. (D) Ectopic expression of Cdc42 reverses Mig-6 inhibitory effect on EGF-induced cell migration. H1299 cells stably expressing Myc-Mig-6 were infected with lentivirus expressing HA-Cdc42 or vector control. H1299 cells stably expressing mutant Myc-Mig-6(4A) was used in parallel. Cell lysates were subjected to western blotting, as indicated. (E) Stable cells were subjected to transwell assays in the presence of EGF for 8 hours. Representative pictures are shown. Scale bars = 100 μm. (F) Results from three independent migration assays were quantitated and presented as means and SE. ***P < 0.001.