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. 2016 Jun 24;7(31):49268–49280. doi: 10.18632/oncotarget.10284

Figure 2. DNAH8 depletion decreases AR-mediated transcriptional activation and is associated with metastatic disease.

Figure 2

(A) and (B) Drosophila S2 cells were transfected with a human AR expression and ARE-luciferase reporter plasmids, along with a control scrambled siRNA (control), AR directed siRNA (AR siRNA), or an siRNA to DNAH8 (CG9492), and luciferase activity was determined after 24 hr in the absence and presence of androgen. **p < 0.01, ***p < 0.001. Error-bars represent standard deviation of three independent experiments. **p < 0.01, ***p < 0.001. (C) Box and whisker plot of DNAH8 mRNA expression in normal (n = 235) compared to primary (n = 235; p = 0.0049), and primary (n = 235) compared to metastatic (n = 44) prostate cancers (p < 0.0001). (D) DNAH5 does not show significantly different mRNA expression in normal prostate, primary tumors, and metastatic tumors (p = 0.78).